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paulnguyen

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So far paulnguyen has created 12 blog entries.

Specificity of recombinant antibodies generated from multiple sclerosis cerebrospinal fluid probed with a random peptide library

Summary: We generated recombinant antibodies (rAbs) from over-represented IgG sequences expressed by single plasma cells from multiple sclerosis (MS) cerebrospinal fluid (CSF). Panning of a phage-displayed random peptide library with the rAbs revealed several specific peptide sequences. Inhibition assays confirmed specific binding of the peptides to the antigen-binding site of the antibody. The native IgG [...]

November 11th, 2005|Uncategorized|

Single-cell RT-PCR analysis of cerebrospinal fluid B cell clonality and immunoglobulin heavy and light chain variable region gene mutation in diffuse large B-cell lymphoma

Summary: Fluorescence-activated cell sorting and reverse transcription-polymerase chain reaction were used to examine cerebrospinal fluid and blood before and during treatment of a patient with diffuse large B cell lymphoma. A monoclonal population of IgM-expressing B cells that used a rearranged VH4 heavy chain variable region and Vκ3 light chain variable region was found. The [...]

January 18th, 2005|Uncategorized|