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Phone: (303) 842-7276

12635 E. Montview Blvd. Suite 131 Aurora CO 80045|

Research & Development

4 09, 2013

Varicella Zoster Virus (VZV)-Human Neuron Interaction

September 4th, 2013|Uncategorized|

Summary: Varicella zoster virus (VZV) is a highly neurotropic, exclusively human herpesvirus. Primary infection causes varicella (chickenpox), wherein VZV replicates in multiple organs, particularly the skin. Widespread infection in vivo is confirmed by the ability of VZV to kill tissue culture cells in vitro derived from any organ. After varicella, VZV becomes latent in ganglionic neurons [...]

18 10, 2011

Intrathecally synthesized IgG in multiple sclerosis cerebrospinal fluid recognizes identical epitopes over time

October 18th, 2011|Uncategorized|

Summary: Intrathecal antibody production manifest as oligoclonal bands (OCBs) is a hallmark of multiple sclerosis (MS). Once present, OCBs can be detected in CSF throughout the lifetime of MS patients. To determine the specificity of the OCBs, we applied CSF IgG obtained from 2 consecutive lumbar punctures of 5 MS patients to screen phage-displayed random peptide [...]

17 04, 2011

Identification of peptide targets in neuromyelitis optica

April 17th, 2011|Uncategorized|

Summary: Neuromyelitis optica (NMO) is an inflammatory demyelinating disease that predominantly affects the optic nerves and spinal cord. Recombinant antibodies (rAbs) generated from clonally expanded plasma cells in an NMO patient are specific to AQP4 and pathogenic. We screened phage-displayed peptide libraries with these rAbs, and identified 14 high affinity linear and conformational peptides. The [...]

16 11, 2010

Peptide reactivity between multiple sclerosis (MS) CSF IgG and recombinant antibodies generated from clonally expanded plasma cells in MS CSF

November 16th, 2010|Uncategorized|

Summary: We employed 19 recombinant antibodies (rAbs) generated from clonally expanded plasma cells, and native IgG from cerebrospinal fluid (CSF) of three multiple sclerosis (MS) patients for panning with phage displayed random peptide libraries. Specific peptide epitopes/mimotopes were identified and characterized. Importantly, peptide–antibody interactions were shared by rAbs and native IgG from the same patient. [...]

9 01, 2009

Antibodies Produced by Clonally Expanded Plasma Cells in Multiple Sclerosis Cerebrospinal Fluid

January 9th, 2009|Uncategorized|

Summary: The cause of multiple sclerosis (MS), a chronic inflammatory demyelinating disease of the central nervous system (CNS), is unknown. Competing theories con- tend that disease is a T-cell–mediated autoimmune response against myelin antigens, infectious, or a virus- triggered immunopathology directed against one or more autoantigens. Clues to the nature of disease may lie in the [...]

18 11, 2008

Identification of measles virus epitopes using an ultra-fast method of panning phage-displayed random peptide libraries

November 18th, 2008|Uncategorized|

Summary: Phage-displayed random peptide libraries, in which high affinity phage peptides are enriched by repetitive selection (panning) on target antibody, provide a unique tool for identifying antigen specificity. This paper describes a new panning method that enables selection of peptides in 1 day as compared to about 6 days required in traditional panning to identify virus-specific [...]

8 10, 2007

Analysis of multiple sclerosis cerebrospinal fluid reveals a continuum of clonally related antibody-secreting cells that are predominantly plasma blasts

October 8th, 2007|Uncategorized|

Summary: Fluorescence-activated cell sorting (FACS) analysis of B cell subtypes in 17 CSF samples from 15 patients with clinically-definite MS revealed that CD19+ B cells accounted for 2 to 11% (mean 5%) and CD138+ cells constituted 0 to 5% (mean 2%) of total CSF lymphocytes. Further stratification of CD138+ cells based on expression levels of CD19 [...]

2 07, 2007

Characterization of phage peptide interaction with antibody using phage mediated immuno-PCR

July 2nd, 2007|Uncategorized|

Summary: Real-time immuno-PCR (RT-IPCR) is a powerful technique that combines ELISA with the specificity and sensitivity of PCR. RT-IPCR of phage-displayed peptides exploits the unique physical associations between phenotype (the displayed peptide) and genotype (the encoding DNA) within the same phage particle. Previously, we identified phage peptides specific for recombinant antibodies (rAbs) prepared from clonally expanded [...]

21 09, 2006

Screening Random Peptide Libraries with Subacute Sclerosing Panencephalitis Brain-Derived Recombinant Antibodies Identifies Multiple Epitopes in the C-Terminal Region of the Measles Virus Nucleocapsid Protein

September 21st, 2006|Uncategorized|

Summary: Infectious and inflammatory diseases of the CNS are often characterized by a robust B-cell response that manifests as increased intrathecal immunoglobulin G (IgG) synthesis and the presence of oligoclonal bands. We previously used laser capture microdissection and single-cell PCR to analyze the IgG variable regions of plasma cells from the brain of a patient [...]

14 08, 2006

Rapid and efficient identification of epitopes/mimotopes from random peptide libraries

August 14th, 2006|Uncategorized|

Summary: Phage-displayed random peptide libraries are important tools in identifying novel epitopes/mimotopes that may lead to the determination of antigen specificity. In this approach, high-affinity phage peptides are enriched by affinity selection (panning) on a monoclonal antibody. To facilitate identification of all potential phage peptides specific for recombinant monoclonal antibodies (rAbs) previously generated from clonally [...]